Genetic Diversity of Toona ciliata Populations based on SSR Markers     

WANG Yang  YUE Dan  LI Xinzhi 《资源与生态学报(英文版)》2020,11(5):466-474

In order to provide a theoretical basis for the protection and development of T. ciliata germplasm resources, we studied the genetic diversity of T. ciliata by using SSR (Simple Sequence Repeat) primers to evaluate the genetic diversity of 192 T. ciliata germplasm samples from 24 populations of 5 provinces. DataFormater, Popgene, NTSYS, TFPGA and other software were used for genetic data conversion, genetic parameter estimation, dendrogram construction and genetic variation analysis. The results showed that: 1) a total of 17 alleles (N_a) were detected in seven pairs of primers, with an average of 2.260 for each primer. Among them, the highest numbers of alleles (4) were detected in primers S11 and S422.The mean value of Nei’s genetic diversity index (H) was 0.4909, the mean value of Shannon information index (I) was 0.7321, and the mean value of polymorphic information content (PIC) was 0.5182. The mean expected heterozygosity (H_e) and observed heterozygosity (H_o) were 0.1055 and 0.4956, respectively. The Nei°s genetic distances of the populations ranged between 0.0002 and 2.6346, and the mean was 0.5477. The average genetic diversity level (H=0.1044) of the 24 populations was lower than that of the species (H=0.4909). 2) The genetic differentiation coefficients (F_st) varied from 0.2374 to 0.9148, with an average value of 0.7727. The mean of population gene flow (N_m) was 0.0735, indicating a low level of genetic exchange between populations, and suggesting that the genetic variation mainly came from within populations. 3) With the UPGMA method, the 24 populations were clustered into 3 groups at Nei’s genetic identity (0.99): the populations from Guizhou Province and Guangxi Zhuang Autonomous Region were clustered into one group, the populations from Hunan Province were in another group, and the populations from Hubei Province were in the third group. The Mantel test analysis showed a significant correlation between Nei’s genetic distance and geographic distance (r=0.6318, P=0.009?0.05). The genetic diversity of the 24 populations of T. ciliata was at a low level. Geographic isolation was the main reason for genetic differentiation among T. ciliata provenances. In the protection of germplasm resources of T. ciliata, emphasis should be placed on breeding genetic resources from the populations with higher genetic diversity (P14, for example). As for the populations with low genetic diversity, an ex-situ protection strategy as well as ecological and timber objectives, should be taken into account to maximize the conservation and utilization of the diversity of T. ciliata.  相似文献   

太平洋真宽水蚤(Eurytemora pacifica) CRH-BP基因克隆及表达分析     

王斌  武敏敏  胡利腾  张建设 《海洋与湖沼》2019,50(1):210-219

促肾上腺皮质激素释放激素结合蛋白(CRH-BP)是一种糖基化蛋白,与促肾上腺皮质激素释放激素(CRH)具有很高的亲和力,对CRH诱导的脑垂体促肾上腺皮质激素(ACTH)的释放起到抑制作用。本研究采用RT-PCR与RACE基因克隆方法首次获得太平洋真宽水蚤(Eurytemora pacifica)CRH-BP基因全长cDNA序列(GenBank登录号:MF289345)1950bp,其中ORF区1245bp编码415个氨基酸,5′非编码区841bp,3′非编码区294bp,理论分子量/等电点为45.816/5.87。生物学序列分析结果表明,太平洋真宽水蚤CRH-BP氨基酸序列与桡足类中近亲真宽水蚤同源性最高;具有6个蛋白修饰位点及蛋白家族标签序列,具有明显的跨膜结构域及信号肽。实时荧光定量PCR分析结果表明,太平洋真宽水蚤CRH-BP基因在盐度、温度及pH环境胁迫中的mRNA表达量均具有一定的时间梯度表达性与浓度梯度表达性特征。本文通过对太平洋真宽水蚤CRH-BP基因结构及不同环境因子刺激下的表达特点的研究为探究桡足类在环境应激、生殖生理及免疫调控等方面奠定了理论基础。  相似文献   

养殖密度对银鲳幼鱼生长、代谢酶活力及其相关基因表达的影响     

倪嘉豪  朱晓静  季益平  周彬  王亚军  徐善良  王丹丽 《热带海洋学报》2020,39(2):54-64

采用为期40d的饲养试验, 探讨养殖密度(30尾·m^-3、60尾·m^-3和90尾·m^-3)对初始平均体质量为(3.88±0.72)g的银鲳幼鱼生长、肝脏和肾脏中谷草转氨酶(AST)、谷丙转氨酶(ALT)、乳酸脱氢酶(LDH)的酶活力以及相应基因mRNA表达的影响。结果显示: 1) 60尾·m^-3组银鲳幼鱼的增重率和特定生长率分别为(235.19±10.23)%和(4.03±0.10)%, 显著高于30尾·m^-3组(p<0.05), 而与90尾·m^-3组无显著性差异(p>0.05); 2) 90尾·m^-3组银鲳幼鱼的酶活力变化最显著, 30尾·m^-3组的变化幅度最小, 60尾·m^-3组介于两者之间; 3) 肝脏和肾脏中LDH、ALT、AST酶活力均呈先升高后降低的趋势, 其中肝脏中LDH、ALT、AST酶活力高峰分别出现在6h、10d和10d, 是初始值的2倍、4.3倍和2倍, 肾脏中3种酶活力峰值则分别出现在1d、3d和5d, 是初始值的2.1倍、2.2倍和3.1倍; 4) AST、ALT、LDH等3种酶基因mRNA表达量变化规律与各自酶活力变化规律一致。综上所述, 养殖密度60尾·m^-3时可促进银鲳幼鱼生长, 90尾·m^-3高密度养殖会导致银鲳幼鱼额外能量的需求增加, 糖异生相关基因mRNA表达量上调使肝脏、肾脏中AST、ALT、LDH酶活力显著升高, 最终出现生长变慢的情况。  相似文献   

海南岛海洋绿藻新记录种未氏仙掌藻(Halimeda velasquezii)的形态学与分子系统学分析     

孟天  陈佐  朱军  邹潇潇  符青艳  鲍时翔 《热带海洋学报》2020,39(6):114-121

2019年4月在海南岛冯家湾潮间带的野外调查过程中采集到一种仙掌藻, 利用传统形态分类学与分子系统分类学结合的方法, 将该种鉴定为未氏仙掌藻Halimeda velasquezii W.R. Taylor。该种的形态特征为藻体呈绿色钙化, 直立生长, 固着器单一较小, 由横卵形或肾形节片连接形成, 节片表面观形态为近圆形, 内部为管状髓丝, 节点处髓丝多发生侧面成对融合, 皮层下髓丝末端无囊状膨大。1,5-二磷酸核酮羧化酶/氧化酶大亚基基因(ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit, rbcL)的序列分析结果显示, 在系统发育树上, 本研究样本与印度洋-太平洋地区产的未氏仙掌藻处于同一分支, 且自展支持值为100, 碱基基因差异为9bp (0.69%)。该种在海南岛海域首次被发现, 为新记录种。本研究同时首次提取并分析了中国种群的rbcL基因序列, 丰富了该种的中国种群的生态学知识, 扩展了其地理分布范围, 对了解海南岛海域海藻资源的生物多样性及其分布特征具有重要意义。  相似文献   

Biodiversity and environmental characteristics of the bycatch assemblages from the tropical tuna purse seine fisheries in the eastern Atlantic Ocean          下载免费PDF全文

Nerea Lezama‐Ochoa  Hilario Murua  Jon Ruiz  Pierre Chavance  Alicia Delgado de Molina  Ainhoa Caballero  Igor Sancristobal 《Marine Ecology》2018,39(3)

The impact of human activities such as fishing has been identified as a main factor in diversity loss in the open ocean. This paper studies the diversity patterns and environmental characteristics of the bycatch assemblages in Fish Aggregating Devices (FADs) and Free School sets (sets made on schools of tuna) from the tropical tuna purse seine fishery in the eastern Atlantic Ocean (35°W–15°E and 20°N–15°S). Data were collected from scientific observer programmes carried out between 2003 and 2011 on board Spanish and French fleets. The results showed different structure and diversity patterns of the bycatch assemblages depending on the fishing mode, with higher number of species and diversity found in FAD sets than in Free School sets. Bycatch assemblages showed preferences for specific oceanographic characteristics of the Atlantic Ocean, such as the equatorial and seasonal coastal upwelling systems, the Cape Lopez front system and the Guinea dome. The type of set and sea surface temperature play an important role to describe the diversity patterns of these species. These results confirm the importance of integrating different methods to study the marine ecosystem towards the correct implementation of the Ecosystem Approach to Fishery Management (EAFM).  相似文献   

海洋蛭弧菌DA5全基因组测序及序列分析     

陆友云  薛明  李志桦  温崇庆 《热带海洋学报》2018,37(6):112-119

为深入挖掘和利用海洋蛭弧菌及其基因资源, 本研究利用Illumina HiSeq测序平台对一株属于嗜盐噬菌弧菌(Halobacteriovorax)的海洋蛭弧菌DA5进行了全基因组测序, 对基因组进行组装、基因预测和功能注释, 并与其他8株蛭弧菌进行了比较基因组分析。结果显示: DA5基因组大小为3.27Mb, GC含量为36.5%, 预测编码基因3175个。在DA5基因组中注释到303个基因具有直系同源蛋白簇分类, 与代谢通路相关基因1239个。比较基因组分析表明: DA5符合海洋蛭弧菌基因组的基本特征, 与其他8株蛭弧菌共有467个同源基因家族, 而DA5特有基因为266个。DA5中与细胞运动相关基因62个, 其中编码甲基受体趋化蛋白、鞭毛蛋白、鞭毛马达及其开关蛋白基因均与嗜盐噬菌弧菌BAL6_X的对应基因亲缘关系最近。对DA5全基因组序列的注释和功能分析, 为深入研究其捕食特性和作用机制, 并更有效地利用其防控海水养殖细菌病害提供了基础。  相似文献   

刺参GPR54-like受体基因克隆及特征分析     

于秋寒  平洪领  闫允君  史会来  吕振明  杨静文 《海洋科学》2018,42(3):92-100

采用c DNA末端快速扩增技术(rapid amplification of c DNA ends,RACE)克隆了刺参GPR54-like(Apostichopus japonicus,Aj GPR54-like,简称Aj GPR54L)基因c DNA全长,对Aj GPR54L全长c DNA序列进行了生物信息学分析;通过绿色荧光蛋白(GFP)融合表达,对其在HEK293细胞中进行了亚细胞定位分析;采用实时荧光定量PCR技术(q RT-PCR)检测Aj GPR54L在组织中的表达。结果表明:该基因全长1454 bp,包含993 bp的开放阅读框(open reading frame,ORF),编码330个氨基酸,191 bp的5′非翻译区(UTR)和270 bp的3′-UTR.预测蛋白质的相对分子质量为37.83 ku、等电点(p I)为9.81;预测蛋白序列分析显示Aj GPR54L具有GPCR家族蛋白典型的七次跨膜结构,包含1个预测的N-连接糖基化位点、5个Asn-Xaa-Ser/Thr序列子和34个磷酸化位点,无信号肽酶切位点;与已鉴定的GPR54s氨基酸序列同源性分析表明,Aj GPR54L与斑马鱼GPR54序列相似性最高,为30.0%;共聚焦显微镜检测显示Aj GPR54L-EGFP可定位于细胞膜表面;基因表达定量分析结果显示,Aj GPR54L在刺参呼吸树、消化道、体壁、肌肉、神经环中均有表达分布,且神经环中表达量显著高于其他组织。  相似文献   

Planktonic rotifers in mining lakes in the Silesian Upland: Relationship to environmental parameters   总被引：1，自引：0，他引：1  

Irena Bielańska-Grajner  Anna G?adysz 《Limnologica》2010,40(1):67-72

The effects of coal-mine waters on the structure and the density of planktonic rotifer communities were studied in lakes formed as a result of subsidence due to mining (collapse of underground tunnels). Water samples were collected according to the standard methods from four mining lakes in Tychy-Czu?ów (near Katowice, southern Poland), twice a month from June till October in 2004-2006. Two of the studied lakes were fed by mine waters but differed in flow rate, conductivity, water pH, and the amount of aquatic vegetation. The effects of those factors on the planktonic rotifer communities were analysed. In total, 83 species and forms of rotifers were recorded. The highest diversity of rotifers was found in the lakes with the largest patches of aquatic vegetation, while the highest density of rotifers was observed in the smallest and the most eutrophicated lake. High-water conductivity limited both species diversity and densities of rotifers. The rotifer communities of the studied lakes differed significantly in species diversity, although all the lakes were dominated by eurytopic species: Keratella cochlearis, Brachionus angularis, Brachionus calyciflorus, and Polyarthra sp. In the largest lake, characterized by the lowest conductivity and pH, the superdominant was Polyarthra luminosa, which in the other lakes was rare or absent. Higher conductivity was correlated with a lower density of P. luminosa, Brachionus diversicornis, K. cochlearis f. tecta, and a higher density of B. calyciflorus. In the lakes with the largest patches of aquatic vegetation, conductivity affected the number of dominant rotifer species. Apparently no other abiotic factors (pH, phosphates, and nitrates) affected the rotifer communities.  相似文献   

古村落景观基因图谱的平台系统设计     

胡最  刘沛林  申秀英  刘晓燕  邓运员  陈影 《地球信息科学学报》2010,12(1):83-88

我国古代先进的社会经济文化孕育了为数众多的古村落。本文分析了近些年我国古村落研究的基本特点,并以"文化景观基因学说"分析了古村落景观基因信息图谱平台构建的内容与意义,以及其关键技术与解决方案。同时,讨论了该程序的基本框架和主要功能、需求分析、功能模块划分与实现思路。最后,以湖南江永女书为例,对古村落景观基因信息进行了提取实验。  相似文献   

Distribution and Diversity of Candida tropicalis Strains in Different Marine Environments     

YAN Kuiran  ZHANG Ying  CHI Zhenming 《青岛海洋大学学报(英文版)》2010,(2):139-144

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